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Study of the effect of fertilizer containing wheat bran on lettuce growth
Smrčková, Kamila ; Pořízka, Jaromír (referee) ; Diviš, Pavel (advisor)
The aim of the work was to perform growth experiments on a laboratory scale with soil, which was enriched with 2,5 vol. % of wheat bran/coffee grounds/biochar and to study soil and growth characteristics on Lactuca sativa L. The coffee grounds were added raw or modified by oxidation/extraction. Phytotoxic properties of coffee grounds were confirmed by germination tests, although there was an effort to reduce the content of phenolic substances by oxidation /extraction. Soil additives caused an increase in content of organic matter, water capacity, pH and conductivity of all mixtures compared to the control soil. Elemental analysis of soils before cultivation did not show any positive effect on the content of mineral elements compared to the control soil. On the other hand, higher mineral (P, Mg, Ca, K, Mn, Fe, Cu and Al) content compared to soil was recorded cultivation experiments. Chlorophyll a was the most abundant in cultivated plants and reached the lowest concentration in plants grown in a mixture with EXKS. Salads differed in mineral content, too. Salads with the highest content of phosphorus were growed on the mixture BU+OT+PŮDA. On the contrary, content of potassium is salads was negatively affected by mixture with EXKS. Microelements (Fe, Zn, Al, Cu, Cr and Mn) were most affected in the salad by the addition of raw coffee grounds and OXKS2.
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Study of drugs intake by lettuce
Amrichová, Anna ; Zlámalová Gargošová, Helena (referee) ; Řezáčová, Veronika (advisor)
The aim of this thesis is to analyze the content of selected groups of drugs in cultivated lettuce. The theoretical part includes a chapter dedicated to the drugs used, their use, physicochemical properties, and their impact on the environment. Other chapters focused on analytical methods for determining drugs, hydroponic cultivation of lettuce, and some parameters of ecotoxicological tests. The experimental part began with the optimization of the QuEChERS extraction procedure. The optimized procedure was then applied to hydroponically grown samples of roots and leaves of lettuce containing selected groups of drugs, which were subsequently analyzed using liquid chromatography – mass spectrometry. The experimental part also addressed the ecotoxicological impact of different drug concentrations on hydroponically grown lettuce.
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Využití symbiotických mikroorganismů a podpůrných nanočástic při zvyšování odolnosti zeleniny v podmínkách vodního stresu
Stašková, Nicole
This thesis deals with the influence of symbiotic microorganisms and supporting nanoparticles on vegetable. Lettuce (Lactuca sativa var. capitata L.), variety "ALAMER" was chosen to be the model vegetable. Symbiotic microorganisms Rhizophagus irregularis and Bacillus subtilis were applied to lettuce plants, and GO-N-1 nanoparticles containing graphene oxide and dicyandiamide were applied to lettuce leaves. The following parameters were evaluated on lettuce plants: height of the above-ground part of plants, weight of the above-ground part of the plants, normalized differentiation index (NDVI), quantum yield of photosynthesis (QY), nitrate content in fresh lettuce leaves and the occurance of mycorrhizical structures.
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Analysis of vegetable quality in aquaponic production system
Botek, Petr
Aim of this study was to closely observe the aquaponic production of lettuce and based on regular measurements of water and plant parameters evaluate these changes and their effect on final product. During two subsequent experiments, effect of orange light intensity (1 and 8 % of total PAR) was examined. Each treatment examined red and green commercial lettuce cultivar in terms of morphological and physiological traits and their correlation to light and composition of nutrients in aquaponic water. Results of in situ measurements of photosynthesis (OJIP, QY, NDVI) and laboratory analysis of harvested plants were evaluated. Increased orange light stimulated assimilation of TAC by 2-fold on average. TAC was also positively correlated to phosphate and calcium concentrations in water. Many responses were cultivar specific. Changes in orange intensity stimulated zinc uptake. Prior to visual deterioration of plant fitness, NDVI values showed decline- demonstrating good ability to detect stress. OJIP values enables in depth evaluation of photosynthetic process at different fitness stage. Changes in orange part of spectrum proved to be trigger of plant assimilation and can be further utilized to improve plant antioxidant capacity. The orange colour has also potential of stimulating heavy metal uptake, of which further research is needed.
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Study of the effect of fertilizer containing wheat bran on lettuce growth
Smrčková, Kamila ; Pořízka, Jaromír (referee) ; Diviš, Pavel (advisor)
The aim of the work was to perform growth experiments on a laboratory scale with soil, which was enriched with 2,5 vol. % of wheat bran/coffee grounds/biochar and to study soil and growth characteristics on Lactuca sativa L. The coffee grounds were added raw or modified by oxidation/extraction. Phytotoxic properties of coffee grounds were confirmed by germination tests, although there was an effort to reduce the content of phenolic substances by oxidation /extraction. Soil additives caused an increase in content of organic matter, water capacity, pH and conductivity of all mixtures compared to the control soil. Elemental analysis of soils before cultivation did not show any positive effect on the content of mineral elements compared to the control soil. On the other hand, higher mineral (P, Mg, Ca, K, Mn, Fe, Cu and Al) content compared to soil was recorded cultivation experiments. Chlorophyll a was the most abundant in cultivated plants and reached the lowest concentration in plants grown in a mixture with EXKS. Salads differed in mineral content, too. Salads with the highest content of phosphorus were growed on the mixture BU+OT+PŮDA. On the contrary, content of potassium is salads was negatively affected by mixture with EXKS. Microelements (Fe, Zn, Al, Cu, Cr and Mn) were most affected in the salad by the addition of raw coffee grounds and OXKS2.
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